hPSC-PRs survive and mature long-term in vivo. This study demonstrates the use of a novel, biocompatible PDMS scaffold to successfully deliver hPSC-PRs in an organized manner into the subretinal space of degenerate rat retinas. This was largely mitigated in transplants with scaffolds seeded with day 115 hPSC-OVs, where proliferation was low and high percentages of transplanted PRs were maintained out to 6 months, the latest time point examined. Proliferation of residual retinal progenitor cells (RPCs) and ectopic cell migration was also noted in transplants with scaffolds seeded with day 70 hPSC-OVs. In vivo, PR precursors mature to both rods and cones, which form outer segments and express key synaptic markers. A large percentage of surviving donor cells differentiated into mature PRs during this period. Statistical analyses were performed with GraphPad Prism.ĭonor cells transplanted on PDMS scaffolds survived and were observed in the subretinal space at both 2 and 6 months post-transplantation. Confocal images were quantified using Columbus image analysis software.
Eyes collected 2 or 6 months post-transplant (n=4 eyes each) were analyzed for PR survival, differentiation, and synaptic marker expression. After 1 month, punches from seeded PDMS scaffolds were transplanted into the subretinal space of adult immune-compromised S334ter rats.
Dissociated hPSC-OVs were seeded onto a micro-patterned polydimethylsiloxane (PDMS) scaffold at day 70 or day 115, which represent early and mid-stages of PR differentiation, respectively. To determine the impact of donor cell maturity on graft survival and cell differentiation in vivo, cells were transplanted at early- and mid-stages of differentiation.Īn hPSC CRX +/tdTomato reporter line was used to produce optic vesicle-like structures (OVs). For this study, we transplanted a novel biocompatible scaffold seeded with human pluripotent stem cell (hPSC)-derived PRs in the S334ter rat model of retinitis pigmentosa.
Disorganization of retinal grafts and reflux of donor cells during surgery can prevent optimal cell delivery in photoreceptor (PR) transplantation.
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